How To Quit Smoking
Saturday, July 12th, 2008If your reading this document then I would assume that you are eager if not desperate to rid yourself of the addiction that has permeated probably almost every ...
Archive for July, 2008
If your reading this document then I would assume that you are eager if not desperate to rid yourself of the addiction that has permeated probably almost every ...
Polymerase chain reaction (PCR)
Thursday, July 3rd, 2008DNA is mixed with short (10–20 base) single-stranded oligonucleotide primers that are complementary to the 5′ and 3′ ends of the sequence to be amplified. The mixture is heated to dissociate or “melt” all double-stranded DNA, and then cooled to permit the primers to anneal to their complementary sequences on the ...
NUCLEOTIDE SEQUENCING
Thursday, July 3rd, 2008The nucleotide sequence of a gene’s coding region encodes the amino acid sequence of its protein. This means that even in the absence of any knowledge about a gene’s protein, we can predict the structure of that protein given the nucleotide sequence of the gene. How can the nucleotide sequence ...
PULSED-FIELD GEL ELECTROPHORESIS
Thursday, July 3rd, 2008One application for Southern blotting is the direct demonstration of physical linkage between two genes. If two different gene probes were to hybridize to the same restriction fragment in a Southern blot, this would prove that the loci of the two genes were closely linked. Unfortunately, eukaryotic genetic linkages ordinarily ...