Sunday, June 1st, 2008
SOUTHERN BLOTTING
One of the most useful techniques for analyzing a gene at the level of genomic DNA is Southern blotting, named for its originator, E.M. Southern.18 In general, it allows one to determine whether specific nucleotide sequences in a cloned probe are present in a sample of genomic DNA. The ...
Posted in Cancer Biology, Cancer Medicine | No Comments »
Sunday, June 1st, 2008
In eukaryotic chromosomes, individual molecules of DNA are several million base pairs long. Because these molecules are far too large to analyze directly, scientists are usually interested in cutting DNA into fragments of manageable size. Fortunately, for molecular biologists, bacteria have evolved a highly diverse set of enzymes, the “restriction ...
Posted in Cancer Biology, Cancer Medicine | No Comments »